A Rapid Polarographic Microdetermination of Glucose with Glucose Oxidase

Polarographic method for rapid microdetermination of cholesterol with cholesterol esterase and cholesterol oxidase.

Cholesterol concentrations in serum are enzymatically determined rapidly by use of a polarographic oxygen analyzer with a circuit modified to record simultaneously the amount and rate of oxygen consumption. The final assay system, assessed from the oxygen consumption value that we found to be optimum, consists of 1 ml of sodium phosphate buffer (0.6 mol/liter, pH 7.0) containing NaN3 (10 mg/lit...

Preparation of a modified glucose oxidase reagent for the polarographic determinationof glucose with the Beckman "glucose analyzer".

In this modification, glucose oxidase, 140-180 U/ml, is dissolved in acetate buffer (pH 6):ethanol (96%):glycerol (80:10:10 by vol). Iodine and molybdate are used as catalysts, formaldehyde and mercuric iodide as preservatives, and octanol as an antifoaming agent. The reagent is suitable for use in the Beckman "Glucose Analyzer", it is stable at room temperature for at least six months, it is s...

A Simple Image Analysis Method for Determination of Glucose by using Glucose Oxidase CdTe/TGA Quantum Dots

Glucose, as the major energy source in cellular metabolism, plays an important role in the natural growth of cells. Herein, a simple, rapid and low-cost method for the glucose determination by utilizing glucose oxidase and CdTe/thioglycolic acid (TGA) quantum dots (QDs) on a thin layer chromatography (TLC) plate has been described. The detection was based on the combination of the glucose enzym...

A Highly Sensitive Glucose Electrode Using Glucose Oxidase Collagen Film

Glucose Oxidase-Based Glucose-Sensitive Drug Delivery for Diabetes Treatment

The glucose-sensitive drug delivery systems based on glucose oxidase (GOD), which exhibit highly promising applications in diabetes therapy, have attracted much more interest in recent years. The self-regulated drug delivery systems regulate drug release by glucose concentration automatically and continuously to control the blood glucose level (BGL) in normoglycemic state. This review covers th...